Thermo Fisher Scientific LRRC25 Monoclonal Antibody (OTI1D9), TrueMAB
Human LRRC25 단백질을 인식하는 Mouse IgG1 형 단일클론 항체. Western blot, IHC, ICC/IF, Flow cytometry 등에 적합. 동결건조 형태로 제공되며, 재구성 및 접합 실험에 용이. RLR 및 NF-κB 신호 억제 관련 연구에 활용 가능.
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Thermo Fisher Scientific LRRC25 Monoclonal Antibody (OTI1D9), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1D9 |
| Immunogen | Full-length human recombinant protein of LRRC25 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
LRRC25 plays a role in inhibiting the RLR-mediated type I interferon signaling pathway by targeting DDX58/RIG-I for autophagic degradation. It interacts with ISG15-associated DDX58, facilitating the interaction between DDX58 and the autophagic receptor p62/SQSTM1 to mediate selective autophagic degradation. LRRC25 also contributes to inhibition of NF-κB signaling and inflammatory response by promoting degradation of p65/RELA.
[Source: UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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