Thermo Fisher Scientific LRRC25 Monoclonal Antibody (OTI1D4), TrueMAB
LRRC25 단백질을 인식하는 Mouse monoclonal antibody로, Western blot, IHC, ICC, Flow cytometry에 적합합니다. Lyophilized 형태로 제공되며, PBS buffer와 trehalose로 안정화되어 있습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1D4 |
| Immunogen | Full length human recombinant protein of human LRRC25 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform additional desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
LRRC25 plays a role in inhibiting the RLR-mediated type I interferon signaling pathway by targeting DDX58/RIG-I for autophagic degradation.
It interacts with ISG15-associated DDX58 to promote interaction with the autophagic cargo receptor p62/SQSTM1, mediating DDX58 degradation via selective autophagy (PubMed:29288164).
Additionally, it inhibits the NF-kappa-B signaling pathway and inflammatory response by promoting degradation of p65/RELA. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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