Thermo Fisher Scientific PADI4 Monoclonal Antibody (OTI6A2), TrueMAB
인간 PADI4 단백질을 인식하는 Mouse IgG1 단일클론 항체로, WB, IHC, ICC/IF, Flow Cytometry에 사용 가능. Lyophilized 형태로 제공되며, PBS buffer에 trehalose 함유. 연구용으로 단백질 시트룰리네이션 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6A2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 299–588 of human PADI4 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Catalyzes the citrullination/deimination of arginine residues in proteins.
Citrullinates histone H3 at Arg-8 and/or Arg-17 and histone H4 at Arg-3, preventing methylation by CARM1 and HRMT1L2/PRMT1 and repressing transcription.
Also citrullinates EP300/P300 at Arg-2142, enhancing interaction with NCOA2/GRIP1.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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