Sartorius Vivacon 2, 10,000 MWCO Hydrosart

100 µl - 2 ml

Vivacon® 2 are centrifugal units for fast DNA concentration and buffer exchange of 2 ml samples. They are also the product of choice for protein fractionation applications. The use of Hydrosart® ultrafiltration membranes results in high recoveries from dilute samples. Complete recovery of the concentrate is guaranteed by a reverse spinning step.

Overview

Vivacon® 2 is optimized for the concentration / recovery and buffer exchange applications of genomic DNA and peptides for samples up to 2 ml initial volume. They are also the product of choice for peptide fractionation applications in this volume range. For optimal performance with highly diluted samples, e.g. forensic specimens, Vivacon 2 is equipped with patented Hydrosart® regenerated cellulose membranes with extremely low unspecific binding properties and high flux. Selected MWCOs are available sterile and DNA free (PCR Grade units). They have been treated with a dual-cycle ethylene oxide (ETO) gas process to ensure they are sterile to a safety assurance level of 106 and free of detectable human genomic DNA contamination. Tests were performed with a qPCR assay over 55 cycles with a verified detection limit of 2 pg.

High recoveries and excellent reproducibility are paired with convenience offered by volume graduation and molecular weight cut-offs printed on individual devices. Vivacon 2 features a horizontal membrane design optimal for DNA fractionation (primer removal) as well as peptide fractionation (Filter Aides Sample Preparation, FASP1). The possibility of a reverse spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations.

1Wisniewski JR, Zougman A, Nagaraj N, Mann M. Universal sample preparation method for proteome analysis (2009). Nat Methods. 6(5):359-62.

• Concentration of up to 2 ml genomic DNA or peptide samples
• Low binding material
• Reverse spin possibility
• MWCO printed on device
• Complete and highly reproducible concentrate recovery
• Selected cut offs available sterile and DNA free* for e.g. forensic applications
• PCR assay over 55 cycles with a verified limit of detection < 2 pg