Thermo Fisher Scientific CD27 Monoclonal Antibody (O323), Super Bright 600, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test
• Publications: View 6 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 615 / 737 / 805
• Brilliant Violet 421 / 480 / 605 / 711
• eFluor 450
• FITC
• NovaFluor Blue, Red, UV, Violet, Yellow series
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 645 / 702 / 780
• Request custom conjugation

Product Specific Information

Description:
The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and most mature T cells, and is upregulated upon T-cell stimulation. CD27 binds CD70 and mediates T cell–B cell interactions.

Applications Reported:
Suitable for flow cytometric analysis.

Applications Tested:
Pre-titrated and validated for flow cytometry using normal human peripheral blood cells. Recommended usage: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Fluorochrome Information:
Super Bright 600 is a tandem dye excited at 405 nm and emitting at 600 nm. Recommended filter: 610/20 bandpass. Ensure instrument compatibility.

Staining Recommendations:
When using multiple Super Bright dyes, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific interactions.

Light Sensitivity:
Sensitive to photo-induced oxidation. Protect from light.

Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.

Laser Compatibility:
Excitation: 405 nm
Emission: 600 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor receptor (TNFR) superfamily, including CD40 and CD30. It regulates cell proliferation and apoptosis, and its expression is largely confined to lymphocytes. CD27 forms a disulfide-linked homodimer on thymocytes, peripheral T cells, and some B cells. Upon T-cell activation, CD27 expression increases, and soluble CD27 (sCD27, 28–32 kDa) can be detected in plasma and other fluids. sCD27 serves as a prognostic marker for B cell malignancies. CD27-binding protein (SIVA) is associated with CD27-mediated apoptosis.
Diseases related to CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without authorization.

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