Applied Biosystems™

TaqMan™ 2.5X Lyo-Ready 1-step qPCR Master Mix, with excipient

Applied Biosystems TaqMan 2.5X Lyo-Ready 1-Step qPCR Master Mix with excipient is designed to be incorporated directly into a lyophilization자세히 알아보기

Applied Biosystems TaqMan 2.5X Lyo-Ready 1-Step qPCR Master Mix with excipient is designed to be incorporated directly into a lyophilization process. No additional excipients are required.

Primers and probes of your choice are added to the master mix and the mixture is then lyophilized. A recommended lyophilization program is provided to help reduce the amount of time needed to optimize the lyophilization process.All lots of master mix are functionally tested to help ensure lot-to-lot reproducibility. This reagent is manufactured in compliance with ISO 13485 requirements.

Request a sample ›

Benefits of TaqMan 2.5X Lyo-Ready 1-step qPCR Master Mix with excipient include:
• Ready for lyophilization; guidelines available
• Optimized for multiplexing—enables up to five-target detection per reaction
• Tolerance of PCR inhibitors that are typically found in biopharmaceutical, molecular diagnostic, and research applications from both purified samples and crude lysates
• Lot-to-lot consistency

Optimized for lyophilization
TaqMan 2.5X Lyo-Ready 1-step qPCR Master Mix with excipient has been optimized for consistent performance before and after lyophilization (see figure below). Comprehensive lyophilization guidelines are included with the product to help shorten your path from liquid to lyophilized product. The proprietary excipient formulation helps ensure fast re-hydration of the lyophilized product after liquid sample is added.

Optimized for multiplexing
TaqMan 2.5X Lyo-Ready 1-step qPCR Master Mix with excipient has been validated for multiplexing up to five (5) targets in one reaction, allowing for additional assays and/or controls to be run simultaneously for increased efficiency. The figure below illustrates this multiplexing capability by comparing performance between singleplex and multiplex reactions.

Tolerance of inhibitors
The unique proprietary formulation allows robust performance even in the presence of substances that can inhibit PCR, resulting in effective template detection from both purified RNA and crude lysate (see figures below).

Lot-to-lot consistency
TaqMan 2.5X Lyo-Ready 1-step qPCR Master Mix with excipient has been optimized for highly reproducible detection from a wide variety of samples (see figure below). Another figure below shows the consistent Cq results obtained from three unique lots across multiple assays, to help maximize confidence in your results and highlight the exceptional lot-to-lot consistency of this master mix.

사양

검출 방법Primer-probe

형태Liquid

형식Master Mix

PCR 방법1-step RT-qPCR, Multiplex qPCR

중합효소AmpliTaq Fast DNA Polymerase

반응 속도Fast or Standard

패시브 참조 염료None

수량5 mL

용도(애플리케이션)Virus Detection, Gene Expression

호환성Lyophilization

농도2.5X

핫 스타트Built-in Hot Start

반응 수500 x 25 μL Reactions

샘플 종류RNA, DNA

부피1 x 5 mL

Unit SizeEach