Thermo Fisher Scientific CD47 Monoclonal Antibody (2D3), eBioscience

Thermo Fisher Scientific CD47 Monoclonal Antibody (2D3), eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugate Formats

• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• Super Bright 436
• Request custom conjugation

Product Specific Information

The monoclonal antibody 2D3 reacts to CD47, also known as integrin-associated protein (IAP) and neurophilin. CD47 is a glycosylated five-transmembrane protein with a small alternatively spliced cytoplasmic domain. It interacts with SIRP (signal regulator protein) and β3 integrins (CD51/CD61, CD41/CD61) to mediate bidirectional signaling that modifies neural synaptic activity and regulates macrophage phagocytosis. CD47 also acts as a receptor for thrombospondin, influencing T cell activation or apoptosis under high thrombospondin conditions. Expression is found in most hematopoietic cells (T, B cells, monocytes, platelets, erythrocytes) and in non-hematopoietic cells.

Applications Reported: Flow cytometric analysis and immunoblotting (WB) under nonreducing conditions.
Applications Tested: Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended use ≤0.5 µg per test (100 µL final volume), with cell numbers ranging from 10⁵ to 10⁸ per test. Optimize antibody titration for best performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein that interacts with integrins and regulates their functions. Engagement by ligands or counter receptors modulates signaling pathways, including G protein activation and intracellular calcium increase upon adhesion. CD47 serves as a receptor for the C-terminal domain of thrombospondin and plays roles in membrane transport and signal transduction. It is broadly distributed across tissues, reduced on Rh erythrocytes, and acts as a ligand for CD172a (SIRPα), preventing phagocytosis of CD47-positive cells. The CD47–CD172a interaction affects cell migration, B cell adhesion, T cell activation, chondrocyte mechanotransduction, and neuronal development, especially in synapse-rich brain and retinal regions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.