Thermo Fisher Scientific CRYAB Polyclonal Antibody
Thermo Fisher Scientific의 CRYAB Polyclonal Antibody는 인간, 마우스 등 다양한 종에 반응하며 Western blot, IHC, ICC/IF, IP에 사용 가능합니다. 알파 B 크리스탈린 단백질 검출용으로, 단백질 A로 정제된 액상 항체입니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 | - |
| Immunohistochemistry (IHC) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 1:200 | - |
| Immunoprecipitation (IP) | Assay-dependent | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Hamster, Human, Mouse, Pig, Rabbit, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide: R(163)EEKPAVTAAPKK(175) KLH conjugated, corresponding to human alpha B Crystallin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2229846 |
Product Specific Information
- Expected to cross-react with hamster and rabbit due to sequence homology.
- Does not cross-react with beta Crystallin or alpha A Crystallin.
- Detects a ~22 kDa band.
- Store as a concentrated solution. Centrifuge briefly before opening the vial.
Target Information
Crystallins are the major structural proteins of the vertebrate eye lens, comprising about 95% of total lens protein. They are also found in vertebrate skeletal muscle tissue.
The three main classes of crystallins are alpha, beta, and gamma. Alpha-crystallin, the largest, consists of two gene products: alpha-A and alpha-B.
Alpha-crystallin maintains lens transparency and refractive index, and functions as a molecular chaperone to prevent protein aggregation that can lead to cataracts.
Phosphorylation of alpha-crystallin is associated with cellular stress response and protein maintenance.
Alpha-B crystallin has been linked to Alexander’s disease, where it accumulates in brain cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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