Thermo Fisher Scientific LIN28A Polyclonal Antibody
LIN28A 단백질을 인식하는 Rabbit Polyclonal Antibody로 Western blot, IHC, Flow Cytometry에 사용 가능. 인간, 마우스, 돼지 등과 반응. 항원 친화 크로마토그래피로 정제, PBS/BSA/glycerol buffer에 보관. 줄기세포 연구 및 iPS 세포 생성 관련 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | References |
|---|---|---|
| Western Blot (WB) | 1:500–1:10,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Flow Cytometry (Flow) | 1:50–1:200 | - |
Product Specifications
| Category | Description |
|---|---|
| Species Reactivity | Human, Mouse, Pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LIN28 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792617 |
Product Specific Information
- Keep as a concentrated solution.
- Predicted reactivity: Mouse (98%), Rat (98%), Cat (96%), Pig (96%), Chicken (82%), Sheep (98%), Bovine (96%).
- Positive Control: human ESC, mouse ESC, pig heart tissue.
- Centrifuge briefly prior to opening the vial.
Target Information
Lin28 is a transcription factor first identified for its role in developmental timing in C. elegans.
It plays a critical role in stem cell generation and maintenance, particularly in the creation of induced pluripotent stem (iPS) cells.
Lin28, along with POU5F1, Sox2, and NANOG, can reprogram fibroblasts into iPS cells.
Recent studies show that iPS cells can be generated using plasmid expression of Lin28, Sox2, POU5F1, and c-Myc, improving safety by avoiding viral vectors.
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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