Thermo Fisher Scientific BID Monoclonal Antibody (21F10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

2 µg

-

Product Specifications

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

21F10

Immunogen

Recombinant human protein purified from E.coli(His-Bid)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_1954920

Product Specific Information

A suggested positive control for this product is A431 cells.

Target Information

BID is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. BID is also a pro-apoptotic member of the Bcl 2 family. BID usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.