Thermo Fisher Scientific Cardiac Troponin I Monoclonal Antibody (3D10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

2 µL

-

Product Specifications

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

3D10

Immunogen

Recombinant Human protein purified from E.coli(His-Troponin I(partial))

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_1961145

Product Specific Information

A suggested positive control for this product is human Troponin I protein.

Target Information

Cardiac Troponin I (Cardiac-specific troponin I, TnI, Troponin I) is an integral inhibitory protein in cardiac muscle that exists as part of a complex with troponin C (TnC) and troponin T (TnT). The TnT subunit of troponin binds to tropomyosin-t form, a troponin-tropomyosin complex, anchored in place by the binding of TnI to actin, within muscle thin filaments. Structural change resulting from the binding of calcium to specific sites on the regulatory TnC subunit, releases the inhibitory region of TnI from actin, anabling the attachment of the molecular motor protein myosin, allowing for muscle contraction and hence movement. The measurements of blood TnI and TnT levels is an important diagnostics indicator of heart muscle damage, and can be used to differentiate between angina and myocardial infarction in patients with chest pains. Cardiac Troponin I has also been shown to inhibit angiogenesis in vivo and in vitro. Mutations in the Cardiac Troponin I gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.