Thermo Fisher Scientific Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4 (asm-1))

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,250-1:6,250

View 23 publications 23 publications

Immunohistochemistry (IHC)

-

View 128 publications 128 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1,250-1:10,000

View 5 publications 5 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:1,250-1:6,250

View 34 publications 34 publications

Flow Cytometry (Flow)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Bovine, Chicken, Human, Mouse, Non-human primate, Rabbit, Rat

Published species

Avian, Cat, Dog, Human, Mouse, Pig, Rabbit, Rat, Rhesus monkey, Sheep

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

1A4 (asm-1)

Immunogen

N-terminal decapeptide of alpha-smooth muscle isoform of actin; acetylated at the N-terminus

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10979529

Product Specific Information

MA5-11547 targets Actin Smooth Muscle IF, WB, and IHC (P) applications and shows reactivity with Bovine, Chicken, Human, mouse, Non-human primate, Rabbit, and Rat samples. This antibody detects a non-specific band at approx. 30 kDa in Hela cell lysates.

The MA5-11547 immunogen is n-terminal decapeptide of alpha-smooth muscle isoform of actin; acetylated at the N-terminus.

Target Information

Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. Multiple alternatively spliced variants of smooth muscle actin have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.