Thermo Fisher Scientific Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4 (asm-1)), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

View 16 publications 16 publications

Immunohistochemistry (IHC)

-

View 142 publications 142 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:800

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 25 publications 25 publications

Flow Cytometry (Flow)

-

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Chicken, Human, Mouse, Non-human primate, Rabbit, Rat

Published species

Cat, Dog, Human, Lizard, Mouse, Pig, Rabbit, Rat, Rhesus monkey, Sheep

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

1A4 (asm-1)

Immunogen

N-terminal decapeptide of alpha-smooth muscle isoform of actin; acetylated at the N-terminus

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

40 µg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10981631

Product Specific Information

MA5-11544 targets Actin Smooth Muscle in WB, immunofluorescence and immunohistochemistry applications and shows reactivity with cow, chicken, human, mouse, non-human primate, rabbit, and rat samples.

The MA5-11544 immunogen is n-terminal decapeptide of alpha-smooth muscle isoform of actin; acetylated at the N-terminus.

Target Information

Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. Multiple alternatively spliced variants of smooth muscle actin have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.