Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (EH12.2H7)

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

-

Flow Cytometry (Flow)

1-4 µg/mL

-

Product Specifications

Species Reactivity

Chimpanzee, Cynomolgus monkey, Human, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

EH12.2H7

Immunogen

Recombinant human CD279

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• APC
• FITC
• PE
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2745543

Product Specific Information

This antibody recognizes an extracellular epitope of CD279 / PD-1 (programmed cell death 1), a 55 kDa type I transmembrane protein expressed above all during T cell development, on activated T cells, activated B cells, and activated monocytes.

Target Information

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of danger signals such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1- mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.