Thermo Fisher Scientific c-Myc Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Positive control: Gastric adenocarcinoma
• Cellular location: Nucleus

Target Information

The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is commonly activated in various tumor cells and plays a critical role in cellular proliferation, differentiation, apoptosis, and cell cycle progression.

Phosphorylation of c-Myc at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2, and JNK has been associated with cell proliferation and regulation.

Studies indicate that c-Myc is essential for vasculogenesis and angiogenesis in tumor development. The c-myc oncogene (p62 c-myc) regulates normal cellular proliferation and differentiation, while deregulated expression can induce apoptosis.

Antibodies against c-Myc epitopes recognize overexpressed proteins containing Myc epitope tags fused to either amino- or carboxy-termini of target proteins. c-Myc-, N-Myc-, and L-Myc-encoded proteins function in cell proliferation, differentiation, and neoplastic diseases.

Amplification of the c-Myc gene has been observed in human tumors such as lung, breast, and colon carcinomas, whereas N-Myc amplification is found in neuroblastomas. Translocation of the c-myc locus on chromosome 8 to immunoglobulin loci on chromosomes 14, 2, or 22 is characteristic of Burkitt’s lymphoma and other B-cell lymphoproliferative disorders.

Aberrant c-myc expression occurs in tumors of multiple origins, including colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, and hematopoietic neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.