Thermo Fisher Scientific Rabbit anti-Chicken IgY (H+L) Secondary Antibody, Biotin

Applications

Tested Dilution

Western Blot (WB)

1:10,000 - 1:400,000

Immunohistochemistry (IHC)

1:500-1:5,000

Immunocytochemistry (ICC/IF)

1:500-1:5,000

Flow Cytometry (Flow)

1:200-1:1,000

ELISA (ELISA)

1:20,000-1:400,000

Immunoprecipitation (IP)

1:500-1:5,000

Product Specifications

Species Reactivity

Chicken

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

Biotin Biotin Biotin

View additional formats

• [AP](/antibody/secondary/product-name/Chicken IgY (H--L)/conjugates/AP/host/Rabbit/)
• [FITC](/antibody/secondary/product-name/Chicken IgY (H--L)/conjugates/FITC/host/Rabbit/)
• [HRP](/antibody/secondary/product-name/Chicken IgY (H--L)/conjugates/HRP/host/Rabbit/)
• [Rhodamine](/antibody/secondary/product-name/Chicken IgY (H--L)/conjugates/Rhodamine/host/Rabbit/)
• [TRITC](/antibody/secondary/product-name/Chicken IgY (H--L)/conjugates/TRITC/host/Rabbit/)
• Request custom conjugation

Form

Lyophilized

Concentration

1.3 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.6, with 15mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_228383

Target

IgY

Antibody Form

Whole Antibody

Product Specific Information

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.

Product # 31720 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.

Product # 31720 reacts with the heavy chains of chicken IgY and with the light chains common to most chicken immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.

Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.

Reconstitute with 1.5 mL of distilled water (1.3 mg/mL after restoration).

Country of Origin: USA

Target Information

Anti-Chicken secondary antibodies are affinity-purified antibodies with well-characterized specificity for the chicken immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.