Thermo Fisher Scientific FITC Monoclonal Antibody (1F8-1E4), TRITC

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50-1:200

-

Immunohistochemistry (IHC)

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1:50-1:200

-

Immunoprecipitation (IP)

1:50-1:200

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1F8-1E4

Conjugate

TRITC TRITC TRITC

View additional formats

• Unconjugated
• Request custom conjugation

Excitation/Emission Max

552/578 nm View spectra

Form

Lyophilized

Concentration

1.7 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.6, with 15mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_908075

Product Specific Information

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.

Fluorophore: Tetramethyl rodamine 5 (and 6)-isothiocyanate (TRITC)

Amax = 550 nm; Emax = 570 nm

Fluorophore/Protein Absorbance Ratio: A550/A280 = 0.44

Product #31687 is recommended for use in ELISA, immunocytochemical, immunofluorescent, immunohistochemical, immunoprecipitation and Western blot applications.

Restoration and Storage: Store product at 4°C until opened. Restore with 0.35 mL distilled water. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. Product may be stored for up to 6 weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of 50% glycerol and store at -20°C; or as an alternative, aliquot without glycerol and store at -80º or below, avoiding repeated freezing and thawing.

Country of Origin: USA

Target Information

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.