Thermo Fisher Scientific NLRP3 Polyclonal Antibody
NLRP3 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, 인간 시료에 반응합니다. IHC, ICC, Flow Cytometry 등 다양한 응용에 사용 가능하며, 비결합형 액상 형태로 제공됩니다. 연구용으로 염증 및 면역 반응 관련 연구에 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 4 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (EKPELTVVFEPSW) corresponding to the C-terminus amino acids of NLRP3 (aa 1024–1036) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10985016 |
Product Specific Information
- Predicted to react with bovine and rat based on sequence homology.
- Tested in Peptide ELISA: antibody detection limit dilution 1:128,000.
- Not recommended for Western Blot.
Target Information
This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site domain, and a leucine-rich repeat motif.
The protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, forming part of the NALP3 inflammasome complex.
This complex acts as an upstream activator of NF-kappaB signaling and regulates inflammation, immune response, and apoptosis.
Mutations in this gene are linked to familial cold autoinflammatory syndrome and neonatal-onset multisystem inflammatory disease.
Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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