Thermo Fisher Scientific Bassoon Polyclonal Antibody
Rabbit polyclonal antibody against human Bassoon protein. Validated for WB and IHC with cross-reactivity in mouse and rat. Lyophilized form, reconstitutable in sterile water. Suitable for studying synaptic organization and neuronal function.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:300–1:2,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from amino acid region 1000–1050 of human Bassoon conjugated to blue carrier protein. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 100 µL of sterile water.
- Centrifuge to remove any insoluble material.
- The peptide is homologous in rat and mouse.
- Specificity: BSN.
Target Information
Bassoon is an approximately 420 kDa protein localized at presynaptic nerve terminals, playing a role in the structural and functional organization of the synaptic vesicle cycle. It is not part of any known protein families and shows 96% sequence identity between rat and mouse.
The human BASSOON gene contains:
- Two double-zinc finger motifs
- Three coiled-coil regions
- Two polyglutamine domains
The polyglutamine domains in the C-terminus are noteworthy due to their potential involvement in late-onset neurodegeneration, similar to Huntingtin. Bassoon is concentrated at presynaptic sites opposite postsynaptic densities and colocalizes with GABA(A) and glutamate (GluR1) receptors. It also colocalizes with Piccolo in cultured hippocampal neurons, suggesting roles in synaptic junction organization and cytoskeletal arrangement at neurotransmitter release sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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