
Thermo Fisher Scientific Prohibitin Monoclonal Antibody (PHB, 3194)
인간 Prohibitin 단백질을 인식하는 Mouse 단일클론 항체로, WB, IHC, ICC 등 다양한 응용 가능. 200 µg/mL 농도의 액상 형태이며 Protein A/G로 정제됨. 세포 증식 조절 및 종양 억제 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PHB, 3194 |
| Immunogen | Recombinant human PHB protein fragment (aa167–261) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It acts as a negative regulator of cell proliferation and may function as a tumor suppressor. Mutations in PHB have been associated with sporadic breast cancer.
Prohibitin is expressed as two transcripts with varying lengths of the 3′ untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3′ untranslated region may function as a trans-acting regulatory RNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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