Thermo Fisher Scientific Phospho-IKB alpha (Tyr42) Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Antibody detects endogenous levels of IKB alpha only when phosphorylated at Tyr42.

Target Information

IkB-alpha is a 40 kDa protein that functions to inhibit NF-kappaB activity through protein-protein interaction with NF-kappaB dimers in the cytosol. This interaction masks the nuclear localization sequence of NF-kappaB, preventing its translocation to the nucleus. Various stimuli such as TNF-alpha, IL-1 beta, chemokines, PMA, growth factors, LPS, UV irradiation, viral infection, and other stresses can activate NF-kappaB by inducing degradation of IkB-alpha.
In humans, the gene is located on the q arm of chromosome 14. Activation of NFkB requires phosphorylation of IkB on specific serine residues (Ser32 and Ser36), leading to its degradation. IKK alpha (CHUK) phosphorylates IkB-alpha at these sites, and its activity is stimulated by NFkB-inducing kinase (NIK). The IKK complex consists of IKK alpha, IKK beta, and IKK gamma, each essential for IkB phosphorylation and NFkB activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.