Thermo Fisher Scientific DYKDDDDK Tag Monoclonal Antibody (FG4R), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-Dependent

-

Product Specifications

Species Reactivity

Tag

Published species

Not Applicable

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

FG4R

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865229

Product Specific Information

Description: This FG4R monoclonal antibody reacts with the DYKDDDDDK tag. This motif is frequently fused to the N- or C-terminus of proteins to facilitate purification (e.g., affinity chromatography and immunoprecipitation) and/or detection. This antibody recognizes the DYKDDDDK anywhere within the protein of interest (i.e., N-terminus, C-terminus, or internal).

Applications Tested: This FG4R antibody has been tested by immunoblotting of reduced cell lysate expressing a DYKDDDDK-tagged protein at less than or equal to 1 µg/ mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.