Thermo Fisher Scientific CD3 Monoclonal Antibody (eBioG4.18 (G4.18)), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• FITC
• Functional Grade
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
The eBioG4.18 monoclonal antibody recognizes the rat CD3 protein, a critical component of the T-cell receptor (TCR) complex and a marker of T-cell lineage. CD3 participates in transducing TCR signals leading to IL-2 transcription and clonal expansion.
In vitro, immobilized G4.18 activates T cells, while soluble G4.18 inhibits allogeneic proliferative responses and cytotoxicity. In vivo, it induces long-term tolerance to organ allografts.

Applications Reported:

• Flow cytometric analysis
• Immunohistochemical staining of frozen tissue sections

Applications Tested:

• Flow cytometric analysis of rat splenocytes (≤ 0.25 µg/test; 10⁵–10⁸ cells/test)
• Immunohistochemistry of frozen rat tissue (≤ 10 µg/mL)
  Antibody concentration should be titrated for optimal performance.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD3 is a subunit complex essential for transducing antigen-recognition signals in T cells and regulating TCR surface expression. The CD3 subunits (γ, δ, ε, ζ) are members of the immunoglobulin superfamily encoded on human chromosome 11.
These subunits associate with cytoplasmic signaling molecules via tyrosine-based motifs and play roles in TCR-induced cell activation, proliferation, and survival.
CD3 is expressed on 68–82% of normal peripheral blood lymphocytes and 65–85% of thymocytes but not on B or NK cells.
Defects in CD3 genes are linked to immunodeficiency disorders.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.