Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), Biotin, eBioscience
인간 CD3 복합체의 epsilon 서브유닛을 인식하는 OKT3 단클론 항체로, Biotin 접합형입니다. Flow cytometry 분석에 최적화되어 있으며 강력한 면역억제 특성을 보입니다. 인간 및 마우스 반응성, 고순도 정제, 연구용으로 적합합니다.
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Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), Biotin, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 3 publications |
| Immunohistochemistry (IHC) | - | 3 publications |
| Immunocytochemistry (ICC/IF) | - | 4 publications |
| Flow Cytometry (Flow) | 0.06 µg/test | 34 publications |
| Functional Assay | - | 4 publications |
| Immunomicroscopy (IM) | - | 1 publication |
| T-Cell Activation (TCA) | - | 5 publications |
| Miscellaneous PubMed | - | 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT3 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1234955 |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Formats
- Unconjugated
- Alexa Fluor 488, 700
- APC, APC-eFluor 780
- Brilliant UV 395, 563, 615, 661, 737, 805
- Brilliant Violet 421, 605
- eFluor 450, 506, 660
- FITC
- Functional Grade
- NovaFluor Blue 510, 585
- NovaFluor Yellow 590, 660, 730
- PE, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available upon request
Product Specific Information
The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. It has potent immunosuppressive properties and has been proven effective in the treatment of renal, heart, and liver allograft rejection.
CD3 subunits (gamma, delta, epsilon) are essential for proper assembly and surface expression of the TCR complex. CD3 is expressed by thymocytes and mature T cells. Crosslinking of TCR initiates intracellular signaling leading to activation and proliferation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of normal human peripheral blood cells at ≤ 0.06 µg/test (100 µL final volume). Cell number may vary (10⁵–10⁸ cells/test). Antibody should be titrated for optimal performance.
Target Information
CD3 subunit complex is critical for transducing antigen-recognition signals and regulating TCR surface expression. The CD3 gamma, delta, epsilon, and zeta subunits are structurally related immunoglobulin superfamily members encoded on chromosome 11.
CD3 components associate with intracellular signaling molecules via tyrosine-based motifs. CD3 is expressed on most T cells (68–82% of lymphocytes, 65–85% of thymocytes) and Purkinje cells but not on B or NK cells.
Defects in CD3 genes are associated with CD3 immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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