Thermo Fisher Scientific TNF alpha Monoclonal Antibody (TN3-19.12), eFluor 660, eBioscience
TN3-19.12 단클론 항체로 마우스, 랫트, 토끼 TNF alpha를 인식하며 인간 TNF alpha에는 반응하지 않습니다. eFluor 660 형광체로 결합되어 Flow Cytometry 등 다양한 응용에 적합합니다. 중화능을 가지며 면역조절 연구에 활용 가능합니다.
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Thermo Fisher Scientific TNF alpha Monoclonal Antibody (TN3-19.12), eFluor 660, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 2 publications |
| ELISA | - | View 1 publication |
| Neutralization | - | View 2 publications |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Rat |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TN3-19.12 |
| Conjugate | eFluor™ 660 |
| Excitation/Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11219268 |
Additional Formats Available:
Product Specific Information
The TN3-19.12 monoclonal antibody reacts with mouse, rat, and rabbit tumor necrosis factor alpha (TNF alpha), but not with human TNF alpha. The TN3-19.12 antibody is a neutralizing antibody. Mouse TNF alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells, and NK cells. A 26 kDa form of TNF alpha is expressed as a membrane-bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF alpha have been observed.
Applications Reported: Suitable for intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes. Recommended usage ≤ 0.5 µg/test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test. Antibody should be titrated for optimal performance.
Fluorophore Information:
eFluor® 660 is a replacement for Alexa Fluor® 647, emits at 659 nm, and is excited with the red laser (633 nm). Ensure instrument compatibility.
Excitation: 633–647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
TNF alpha is a multifunctional proinflammatory cytokine belonging to the TNF superfamily. Secreted primarily by macrophages, it binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha regulates immune cells, proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. It exists as multimers but shows a single 17 kDa band under non-reducing SDS-PAGE conditions. Knockout studies in mice suggest neuroprotective roles and tumor necrosis induction. TNF alpha is involved in immune responses to bacterial, viral, parasitic, and fungal infections, as well as tumor necrosis. It is synergistic with interferon-gamma in cytotoxicity and implicated in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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