Thermo Fisher Scientific p115-RhoGEF Polyclonal Antibody
Human p115-RhoGEF 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot 및 Immunoprecipitation에 적합. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 제공. 세포 신호전달 및 Rho GTPase 연구용으로 사용.
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Thermo Fisher Scientific p115-RhoGEF Polyclonal Antibody
Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 275 and 325 of human 115 kDa Rho guanine nucleotide exchange factor (ARHGEF1, UniProt ID: Q92888-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
The recommended shelf life for this product is 1 year from the date of receipt.
Target Information
The Ras superfamily of GTPases, including Ras, Rho/Rac, Sar, Rab, ARF, and Ran subfamilies, regulates various cellular processes such as cytoskeletal rearrangement, nuclear signaling, and cell growth. These GTPases act as molecular switches toggling between active GTP-bound and inactive GDP-bound states, with activation catalyzed by guanine nucleotide exchange factors (GEFs).
Dbl-related proteins, including FGD1, Lsc, RhoGEF p115, Lfc, Lbc, and Brx, share structural similarity and catalyze GEF activity for specific Ras family members. RhoGEF p115 specifically activates Rho GTPases but not Rac, Cdc42, or Ras.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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