Thermo Fisher Scientific CDK9 Recombinant Rabbit Monoclonal Antibody (SD204-07)
인간 CDK9 단백질을 인식하는 재조합 토끼 단클론 항체로, 높은 특이성과 민감도를 제공. Western blot, IHC, ICC/IF 등 다양한 응용 가능. HEK293 세포 발현 시스템 사용, 단일 클론 SD204-07. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SD204-07 |
| Immunogen | Synthetic peptide within Human Cdk9 aa 323–372 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809675 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
CDK9 is a serine/threonine kinase that forms an active holoenzyme complex with Cyclin T1, playing a key role in regulating the eukaryotic cell cycle. It regulates cytokine-inducible transcription networks and promotes RNA synthesis in genetic programs for cell growth, differentiation, and viral pathogenesis.
The CDK9/cyclin-K complex also has kinase activity towards CTD of RNAPII and can substitute for CDK9/cyclin-T P-TEFb in vitro. Chronic activation of CDK9 causes cardiac myocyte enlargement, leading to cardiac hypertrophy and predisposition to heart failure.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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