Thermo Fisher Scientific Phospho-GSK3 alpha/beta (Ser21, Ser9) Monoclonal Antibody (K.857.1)

Applications

Western Blot (WB)

• Tested Dilution: 1:1000

Immunoprecipitation (IP)

• Tested Dilution: 1:50

Product Specifications

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Glycogen synthase kinase-3 (GSK3) is a protein kinase originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. It is involved in regulation of various cellular functions including protein synthesis, cell proliferation, differentiation, microtubule dynamics, and apoptosis.

GSK3’s substrate specificity requires a pre-existing phosphoserine or phosphotyrosine four residues C-terminal to the phosphorylation site. Two isoforms exist — GSK3α and GSK3β — regulated by phosphorylation. Phosphorylation of GSK3β on Ser9 (Ser21 in GSK3α) by protein kinase B (PKB) inactivates the enzyme, mediating growth factor inhibition. Activation occurs via phosphorylation of Tyr216 (Tyr279 in GSK3α).

Activated GSK3β phosphorylates multiple cellular proteins including p53, c-Myc, c-Jun, HSF-1, and cyclin D1. It also phosphorylates tau protein at abnormal sites, contributing to Alzheimer’s disease progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.