Thermo Fisher Scientific NEDD8 Monoclonal Antibody (B.226.2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:300

-

Immunocytochemistry (ICC/IF)

1:100

-

Immunoprecipitation (IP)

1:25

-

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

B.226.2

Immunogen

Synthetic peptide corresponding to amino acids at the amino terminus of human NEDD8 protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NEDD8,uniProtId:Q15843-1,ncbiNodeId:9606,antigenRange:1,antigenLength:81,antigenImageFileName:MA5-15028_NEDD8_Q15843-1_Rabbit.svg,antigenImageFileNamePDP:MA5-15028_NEDD8_Q15843-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

114 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10985618

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other ubiquitin family members, including ubiquitin, SUMO-1, SUMO-2, SUMO-3 and ISG15.

Target Information

NEDD8 is a ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.