Thermo Fisher Scientific HES6 Monoclonal Antibody (OTI2F7), TrueMAB
HES6 단백질을 인식하는 마우스 단클론 항체로 Western blot에 최적화됨. 인간 시료에 반응하며, 비접합 형태로 동결건조되어 제공. 재구성 시 1 mg/mL 농도로 사용 가능. 연구용으로만 사용.
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Applications
- Western Blot (WB): 1:2,000 dilution tested
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2F7 |
| Immunogen | Full length human recombinant protein of human HES6 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
HES family members form complexes with TLE, the mammalian homologue of Groucho, through the carboxy-terminal WRPW motif. This HES/TLE complex binds directly to DNA.
Most HES proteins (HES1, HES5) prefer the N box (CACNAG) over the E box (CANNTG). HES2 binds both N and E boxes, while HES6 does not bind DNA but inhibits HES1 activity, promoting transcription.
Expression patterns:
- HES1, HES2: Various adult and embryonic tissues
- HES3: Cerebellar Purkinje cells
- HES5: Nervous system
- HES6: Brain and limb buds of developing embryos
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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