Thermo Fisher Scientific HES6 Monoclonal Antibody (OTI2F7), TrueMAB

Applications

• Western Blot (WB): 1:2,000 dilution tested

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

HES family members form complexes with TLE, the mammalian homologue of Groucho, through the carboxy-terminal WRPW motif. This HES/TLE complex binds directly to DNA.
Most HES proteins (HES1, HES5) prefer the N box (CACNAG) over the E box (CANNTG). HES2 binds both N and E boxes, while HES6 does not bind DNA but inhibits HES1 activity, promoting transcription.
Expression patterns:

• HES1, HES2: Various adult and embryonic tissues
• HES3: Cerebellar Purkinje cells
• HES5: Nervous system
• HES6: Brain and limb buds of developing embryos

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.