Takara SMART-Seq mRNA LP (with UMIs)

Achieve higher accuracy in full-length mRNA-seq by combining unique molecular identifier (UMI)-based 5` RNA counting with our ultra-sensitive SMART-Seq chemistry for ultra-low input, full-length transcriptome sequencing. The SMART-Seq mRNA LP (with UMIs) kit is optimized to generate high-quality, full-length cDNA directly from ultra-low inputs (10 pg–100 ng of high integrity RNA, RIN≥8, or 1–1000 intact cells).

Achieve higher accuracy in full-length mRNA-seq by combining unique molecular identifier (UMI)-based 5` RNA counting with our ultra-sensitive SMART-Seq chemistry for ultra-low input, full-length transcriptome sequencing. The SMART-Seq mRNA LP (with UMIs) kit is optimized to generate high-quality, full-length cDNA directly from ultra-low inputs (10 pg–100 ng of high integrity RNA, RIN≥8, or 1–1000 intact cells).

To control for PCR errors and amplification biases, the kit uses oligo(dT) priming and incorporates UMIs in the template-switching oligonucleotide (TSO). This provides greater accuracy for gene/transcript counting. The kit relies on SMART (Switching Mechanism at 5` End of RNA Template) cDNA synthesis technology to obtain full-length transcript information, enabling analysis of transcript isoforms, gene fusions, point mutations, etc. Additionally, the TSO incorporates the locked nucleic acid (LNA) technology for more efficient template switching, resulting in higher sensitivity for the identification of higher numbers of genes relative to other methods.

High-quality, Illumina®-compatible libraries are generated in single-tube workflow, which features our patented strategy of fragmentation and stem-loop adapter ligation. Using the Unique Dual Index (UDI) kits (Cat. Nos. 634752–56; sold separately), up to 384 multiplexed, Illumina-ready sequencing libraries can be generated. The kit offers an end-to-end solution from sample to cDNA synthesis, library preparation, and data analysis with our free Cogent NGS bioinformatics pipeline.

This kit is also compatible with miniaturization and automation on liquid handlers for high-throughput sample processing.

If you prefer a random priming approach that will allow you to work with degraded samples, we recommend our Stranded total RNA-Seq kits.

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Overview

• Reliability—higher accuracy for gene/transcript counting with UMIs
• Superior sensitivity and performance for ultra-low inputs—10 pg–100 ng total RNA, or 1–1,000 cells
• Single-tube cDNA synthesis and library prep workflows—minimized sample loss and handling errors
• Greater multiplexing—up to 384 libraries with Unique Dual Index Kits (Cat. No. 634752–56)
• Complete solution—from sample to data with free, user-friendly Cogent NGS analysis tools

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Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Product Components List to determine kit components. Certificates of Analysis and Product Components Lists are located under the Documents tab.