Thermo Fisher Scientific UBXN2B Monoclonal Antibody (OTI1F7), TrueMAB
UBXN2B 단백질을 타겟으로 하는 Mouse IgG1 단일클론 항체. WB, IHC, Flow Cytometry에 적합. Lyophilized 형태로 공급되며, PBS·trehalose buffer에 재구성 가능. ER 및 Golgi 생합성 관련 연구용으로 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F7 |
| Immunogen | Full length human recombinant protein of human UBXN2B produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
UBXN2B (UBX domain-containing protein 2B), also known as NSFL1 cofactor p37 or p97 cofactor p37, is a 331 amino acid protein containing one UBX domain and one SEP domain.
UBXN2B is essential for ER and Golgi biogenesis and maintenance during interphase, and their reassembly at the end of mitosis.
Through interaction with VCP, UBXN2B forms a complex with membrane fusion activity.
It acts as an adapter protein required for Golgi and endoplasmic reticulum biogenesis, with USO1-GOLGA2 tethering and BET1L involvement.
VCPIP1 is also required, though its deubiquitinating activity is not necessary.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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