
Thermo Fisher Scientific F(ab`)2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555
Alexa Fluor 555로 결합된 염소 유래 F(ab’)2 항-토끼 IgG 2차 항체로, WB 및 ICC/IF에 최적화되어 있습니다. 높은 특이성과 낮은 교차 반응성을 가지며, 553/568 nm 형광 특성으로 민감한 신호 검출이 가능합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific F(ab`)2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555
Applications and Tested Dilution
- Western Blot (WB): 1:5,000–1:10,000
- Immunocytochemistry (ICC/IF): 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Alexa Fluor™ 555 |
| Excitation / Emission Max | 553 / 568 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.5 |
| Contains | 5 mM Sodium Azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient |
| RRID | AB_2535851 |
| Target | IgG |
| Cross Adsorption | Against pooled human serum, mouse serum, mouse plasmacytoma/hybridoma proteins, and purified human paraproteins |
| Antibody Form | F(ab`)2 Fragment |
Product Specific Information
Product will be shipped at room temperature.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins. They are useful for detection, sorting, or purification of the specified target.
Secondary antibodies offer increased versatility by enabling use with various detection systems (e.g., HRP, AP, fluorescence). They provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, these antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Formats Available
Alexa Fluor 350, 405, 430, 488, 514, 532, 546, 568, 594, 633, 635, 647, 660, 680, 700, 750, 790
Alexa Fluor Plus 488, 555, 594, 647
AP, APC, Biotin, Biotin-XX, Cyanine3, Cyanine5, DyLight 350–800, FITC, HRP, Pacific Blue, Pacific Orange, PE, PE-Alexa Fluor 610/647, Rhodamine, Rhodamine Red-X, Texas Red, Texas Red-X, TRITC
Custom conjugation available upon request.
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