Thermo Fisher Scientific Phospho-MiTF (Ser180) Polyclonal Antibody
Phospho-MiTF (Ser180) 폴리클로날 항체로, 인산화된 MITF 단백질의 Ser180 부위를 특이적으로 인식합니다. Western blot, IHC, ICC/IF 등 다양한 응용에 적합하며, 토끼 IgG 기반의 액상 항체입니다. 인체, 마우스, 설치류 등 다양한 종에 반응합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human MITF (Accession O75030), corresponding to amino acid residues around phosphorylated Ser180. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816180 |
Product Specific Information
Antibody detects endogenous levels of MITF only when phosphorylated at Serine 180.
Target Information
Mi is a basic helix-loop-helix-leucine zipper (b-HLH-ZIP) transcription factor involved in pigmentation, mast cell, and bone development.
Mutations in Mi cause Waardenburg Syndrome type II in humans. In mice, this leads to loss of pigmented cells in the skin, eye, and inner ear, as well as osteopetrosis and defects in natural killer and mast cells.
Two isoforms of Mi exist, differing by 66 amino acids at the N-terminus. Shorter forms are expressed in melanocytes (52–56 kDa), while longer forms (60–70 kDa) appear in osteoclasts, B16 melanoma cells, mast cells, and heart tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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