
Thermo Fisher Scientific ERCC1 Monoclonal Antibody (OTI3F2), TrueMAB
ERCC1 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western Blot, IHC, ICC/IF, Flow Cytometry에 적합. 인간 시료 반응성, 고순도 친화 크로마토그래피 정제 제품. DNA 복구 관련 연구에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3F2 |
| Immunogen | Full length human recombinant ERCC1 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
ERCC1 is a non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5′ incision during DNA repair. Together with SLX4, it mediates the first step in the repair of interstrand cross-links (ICL). It also participates in the processing of anaphase bridge-generating DNA structures that arise during S or G2 phase and may lead to cytokinesis failure. ERCC1 is required for homology-directed repair (HDR) of DNA double-strand breaks in conjunction with SLX4. (Source: UniProt)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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