Thermo Fisher Scientific PIM2 Monoclonal Antibody (OTI9A5), TrueMAB
인간 PIM2 단백질을 인식하는 Mouse IgG2a 단일클론 항체로, WB, IHC, ICC, Flow, IP 등 다양한 응용에 적합. 동결건조 형태로 제공되며, PBS 버퍼와 trehalose 포함. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
| Immunoprecipitation (IP) | 2 µg/500 µL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI9A5 |
| Immunogen | Full length human recombinant protein of human PIM2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
PIM2 is a proto-oncogene that phosphorylates BAD, partially inhibiting its apoptotic function. PIM proteins (PIM-1, PIM-2, and PIM-3) are oncogene-encoded serine/threonine kinases. PIM-2 shares high homology with PIM-1 and exhibits similar oncogenic functions. It is predominantly expressed in hematopoietic tissues, spleen, thymus, peripheral blood leukocytes, testis, small intestine, and colon. PIM2 expression decreases upon growth factor depletion, and elevated levels have been observed in certain cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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