
Thermo Fisher Scientific NME4 Monoclonal Antibody (OTI2B10), TrueMAB
인간 NME4 단백질을 인식하는 Mouse Monoclonal 항체로, WB, IHC(P), ICC/IF에 적합합니다. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용 가능합니다. 연구용으로 단백질 인산화 및 세포 신호전달 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2B10 |
| Immunogen | Full length human recombinant protein of human NME4 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
NME4 is a protein kinase enzyme that transfers phosphate groups from donors such as ATP to acceptor amino acids in substrate proteins. This process mediates signal transduction in eukaryotic cells, regulating metabolism, transcription, cell cycle progression, cytoskeletal rearrangement, apoptosis, and differentiation.
Protein kinases form one of the largest protein families in eukaryotes, classified into 8 major groups based on catalytic domain sequences of tyrosine (PTK) or serine/threonine (STK) kinases.
The AGC kinase group includes 63 kinases such as PKA, PKG, PKC, RAC/Akt, ARK, and RSK families.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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