Gibco™

RPMI 1640 Medium, powder, HEPES

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a자세히 알아보기

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. Life Technologies offers a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:

With	Without
• L-glutamine	• Sodium Bicarbonate
• Phenol Red
• HEPES

The complete formulation is available.

Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12 and PABA which are not found in Eagles Minimal Essential Medium or Dulbeccos Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco™ RPMI 1640 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, Life Technologies offers an identical Gibco™ RPMI 1640 product made in our Scotland facility (13018-015). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

This product is not used for in vitro diagnostic purpose in some countries.

사양

세포주HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas

세포 유형Leukemic Cells

분류Animal Origin-free

형태Powder

제품 유형RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)

Serum LevelStandard Serum Supplementation

유통 기한36 Months From Date of Manufacture

멸균Sterile-filtered

첨가제 포함Glutamine, HEPES, Phenol Red

첨가제 없음No Sodium Pyruvate, No Sodium Bicarbonate

제조 품질cGMP-compliant under the ISO 13485 standard

제품라인Gibco™

수량10 x 1 L

배송 조건Room Temperature

Unit SizeEach