Thermo Fisher Scientific CD209a Monoclonal Antibody (LWC06), FITC, eBioscience

Applications

Western Blot (WB)

• Tested Dilution: Not specified
• View 1 publication

Flow Cytometry (Flow)

• Tested Dilution: 0.5 µg/test
• View 1 publication

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• PE
• Request custom conjugation

Product Specific Information

The LWC06 antibody was generated by immunization with the recombinant extracellular region of mouse CIRE/DC-SIGN (CD209).
CIRE/DC-SIGN is a 33 kDa type II transmembrane C-type lectin protein expressed on CD8α⁻ dendritic cells and plasmacytoid predendritic cells, and is the closest homologue of human DC-SIGN.
It contains a C-terminal extracellular Carbohydrate Recognition Domain (CRD) that binds mannose and other carbohydrates in a calcium-dependent manner.

• May play a role in T-dendritic cell interactions via ICAM family binding
• Expression varies with host activation state
• Down-regulated in spleen-derived dendritic cells with GM-CSF
• LWC06 does not cross-react with SIGNR1, SIGNR2, SIGNR3, or SIGNR4
• CD209 protein is sensitive to collagenase treatment

Applications Reported

• Flow cytometric analysis

Applications Tested

• Tested by flow cytometric analysis of mouse CIRE/DC-SIGN-transfected CHO cells
• Recommended usage: ≤ 0.5 µg per test (100 µL final volume)
• Cell number: 1×10⁵–1×10⁸ cells/test
• Excitation: 488 nm; Emission: 520 nm; Laser: Blue
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

This gene encodes a transmembrane receptor (DC-SIGN) expressed on dendritic cells and macrophages.
It functions in innate immunity by recognizing a wide range of pathogens, including parasites and viruses.
The protein comprises three domains:

1. N-terminal transmembrane domain
2. Tandem-repeat neck domain (important for homo-oligomerization)
3. C-type lectin carbohydrate recognition domain (CRD)

The extracellular region acts as both a pathogen recognition receptor and a cell adhesion receptor.
Variations in the neck domain repeats affect ligand-binding ability.
DC-SIGN and its homolog L-SIGN differ in ligand-binding and tissue distribution.
Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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