Thermo Fisher Scientific CD209a Monoclonal Antibody (LWC06), FITC, eBioscience
마우스 CD209a(CIRE/DC-SIGN) 단백질을 인식하는 Rat IgG2a 단클론 항체로, FITC 형광 표지되어 있습니다. 유세포분석(Flow Cytometry)에 적합하며, 488 nm 레이저에서 520 nm 파장으로 방출됩니다. 0.5 µg/test 사용 권장, 4°C 암소 보관.
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- 11-2092-82
- 판매단위
- pk
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Applications
Western Blot (WB)
- Tested Dilution: Not specified
- View 1 publication
Flow Cytometry (Flow)
- Tested Dilution: 0.5 µg/test
- View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | LWC06 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_494110 |
Available Formats
Product Specific Information
The LWC06 antibody was generated by immunization with the recombinant extracellular region of mouse CIRE/DC-SIGN (CD209).
CIRE/DC-SIGN is a 33 kDa type II transmembrane C-type lectin protein expressed on CD8α⁻ dendritic cells and plasmacytoid predendritic cells, and is the closest homologue of human DC-SIGN.
It contains a C-terminal extracellular Carbohydrate Recognition Domain (CRD) that binds mannose and other carbohydrates in a calcium-dependent manner.
- May play a role in T-dendritic cell interactions via ICAM family binding
- Expression varies with host activation state
- Down-regulated in spleen-derived dendritic cells with GM-CSF
- LWC06 does not cross-react with SIGNR1, SIGNR2, SIGNR3, or SIGNR4
- CD209 protein is sensitive to collagenase treatment
Applications Reported
- Flow cytometric analysis
Applications Tested
- Tested by flow cytometric analysis of mouse CIRE/DC-SIGN-transfected CHO cells
- Recommended usage: ≤ 0.5 µg per test (100 µL final volume)
- Cell number: 1×10⁵–1×10⁸ cells/test
- Excitation: 488 nm; Emission: 520 nm; Laser: Blue
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
This gene encodes a transmembrane receptor (DC-SIGN) expressed on dendritic cells and macrophages.
It functions in innate immunity by recognizing a wide range of pathogens, including parasites and viruses.
The protein comprises three domains:
- N-terminal transmembrane domain
- Tandem-repeat neck domain (important for homo-oligomerization)
- C-type lectin carbohydrate recognition domain (CRD)
The extracellular region acts as both a pathogen recognition receptor and a cell adhesion receptor.
Variations in the neck domain repeats affect ligand-binding ability.
DC-SIGN and its homolog L-SIGN differ in ligand-binding and tissue distribution.
Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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