Thermo Fisher Scientific CHEK2 Monoclonal Antibody (PCRP-CHEK2-1A4)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Antibody is stable for 24 months.
• Positive Control: HeLa or HCT 116 cells; human testis, spleen, or colon.
• Cellular Localization: Nucleus.
• Specificity Comments:
  Recognizes a serine/threonine protein kinase required for checkpoint control mediating cell cycle arrest, activation of DNA repair, and apoptosis.
  In response to DNA damage and replication blocks, cell cycle progression halts through regulation of key cell cycle proteins.
  The encoded protein acts as a checkpoint regulator and putative tumor suppressor, containing a forkhead-associated domain essential for activation after DNA damage.
  When activated, it inhibits CDC25C phosphatase, preventing mitotic entry, and stabilizes p53, leading to G1 arrest.
  Also interacts with and phosphorylates BRCA1, aiding survival after DNA damage.
  Mutations are linked to Li-Fraumeni syndrome and may predispose to sarcomas, breast cancer, and brain tumors.
• Predicted Reactivity: Mouse and Rat.

Target Information

CHEK2 (CHK2) is a serine/threonine kinase involved in the DNA damage checkpoint pathway.
Mutations in CHK2 are associated with cancer susceptibility.
CHK2 phosphorylates several modulators of cell cycle control, including tumor suppressor proteins such as p53 and BRCA1, contributing to DNA repair and cell survival.
It functions as a putative tumor suppressor, with mutations linked to Li-Fraumeni syndrome and predisposition to various cancers including sarcoma, breast, and brain tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.