Thermo Fisher Scientific CD209 (DC-SIGN) Monoclonal Antibody (eB-h209), FITC, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test
• Publications: View 9 publications

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The eB-h209 monoclonal antibody reacts with human CD209 (DC-SIGN), a 44 kDa type II transmembrane protein. DC-SIGN contains a C-type lectin binding domain and binds ICAM-3, ICAM-2, and HIV virus. It is expressed on human dendritic cells and may function as a receptor for HIV capture and transmission to T cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested on cultured human dendritic and peripheral blood cells
• Recommended Use: 5 µL (0.5 µg) per test (in 100 µL final volume, 10⁵–10⁸ cells/test)
• Excitation: 488 nm
• Emission: 520 nm
• Laser: Blue Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

The CD209 gene encodes a transmembrane receptor known as DC-SIGN, expressed on dendritic cells and macrophages. It plays a role in innate immunity by recognizing a wide range of pathogens including parasites and viruses. The protein has three domains: a transmembrane domain, a tandem-repeat neck domain, and a C-type lectin carbohydrate recognition domain.

The extracellular region functions as both a pathogen recognition receptor and a cell adhesion receptor. The neck region mediates homo-oligomerization, enhancing multivalent ligand binding. Variations in the neck domain affect ligand affinity. CD209 is closely related to L-SIGN (GeneID 10332), differing in ligand-binding and expression patterns. Alternative splicing produces multiple variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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