Thermo Fisher Scientific MMP9 Monoclonal Antibody (L51/82), FITC

Applications and Tested Dilution

Product Specifications

Additional Formats

• APC (Cat. No. MA5-45511)
• PerCP (Cat. No. MA5-45513)
• Custom conjugation available upon request

Product Specific Information

1 µg/mL of MA5-45512 was sufficient for detection of MMP9 in 20 µg of COS-1 cell lysate transfected with human MMP9 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 92 kDa (pro form) and 82 kDa (active form).
This antibody was formerly sold as clone S51-82.

Target Information

MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase belonging to a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins.
MMP9 is secreted as a 92 kDa zymogen; cleavage yields an active 82 kDa enzyme.
It contains:

• A gelatin-binding domain with three fibronectin type II units
• A catalytic domain with a zinc-binding site
• A proline-rich type V collagen-homologous domain
• A hemopexin-like domain

MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts, and endothelial cells. It plays roles in:

• Inflammatory responses
• Tissue remodeling and wound healing
• Tumor growth and metastasis

MMP9 contributes to skin carcinogenesis and degrades type IV and V collagens.
Studies suggest involvement in IL-8-induced mobilization of hematopoietic progenitor cells and tumor-associated tissue remodeling.
Elevated MMP9 levels are associated with idiopathic atrial fibrillation and aortic aneurysm progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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