Thermo Fisher Scientific MMP9 Monoclonal Antibody (L51/82), APC
MMP9 단백질 검출용 APC 결합 단일클론 항체로, 인간·마우스·랫트 시료에 반응합니다. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용 가능. 단백질 G 정제, 1 mg/mL 농도, 4°C 보관. MMP9의 전구체 및 활성형(92/82 kDa)을 검출합니다.
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Thermo Fisher Scientific MMP9 Monoclonal Antibody (L51/82), APC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | L51/82 |
| Immunogen | Fusion protein amino acids 1–708 (full length) of rat MMP9 |
| Conjugate | APC |
| Excitation/Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 2.48 mM MES / 95.46 mM phosphate, pH 7.4, with 2 mM EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931965 |
Product Specific Information
- 1 µg/mL of MA5-45511 detects MMP9 in 20 µg of COS-1 cell lysate transfected with human MMP9 by colorimetric immunoblot using goat anti-mouse IgG:HRP as secondary antibody.
- Detects approximately 92 kDa (pro form) and approximately 82 kDa (active form).
- Formerly sold as clone S51-82.
Target Information
MMP9 (matrix metallopeptidase 9) is a zinc and calcium-dependent endopeptidase that degrades extracellular matrix proteins. It is secreted as a 92 kDa zymogen; cleavage produces an active 82 kDa enzyme. MMP9 contains:
- Gelatin-binding domain (three fibronectin type II units)
- Catalytic domain with zinc-binding site
- Proline-rich type V collagen-homologous domain
- Hemopexin-like domain
Produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts, and endothelial cells, MMP9 plays roles in:
- Inflammatory responses
- Tissue remodeling and wound healing
- Tumor growth and metastasis
MMP9 contributes to skin carcinogenesis and degrades type IV and V collagens. It is implicated in hematopoietic progenitor cell mobilization, tumor-associated tissue remodeling, idiopathic atrial fibrillation, and aortic aneurysm progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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