Thermo Fisher Scientific VPS53 Polyclonal Antibody
Thermo Fisher Scientific VPS53 폴리클로날 항체는 인간, 마우스, 랫트에 반응하며 Western blot 및 Immunocytochemistry에 적합합니다. 항원 친화 크로마토그래피로 정제된 액상 형태로 제공되며, 2–8°C에서 보관 가능합니다. HIV 연구 및 GARP 복합체 연구에 활용됩니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–1 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | 18 amino acid peptide from near the carboxy terminus of human VPS53 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11151973 |
Product Specific Information
- A suggested positive control is 293 cell lysate.
- PA5-20548 can be used with blocking peptide PEP-0668.
Target Information
The sorting of acid hydrolases to lysosomes relies on mannose 6-phosphate receptors that cycle between the trans-Golgi network (TGN) and endosomes.
The maintenance of this cycle requires the function of the mammalian Golgi-associated retrograde protein (GARP) complex, which is composed of three subunits: VPS52, VPS53, and VPS54.
Depletion of any of these proteins impairs retrograde transport of multiple TGN proteins.
VPS53 was identified as an HIV dependency factor (HDF) and plays a role in viral entry, suggesting its potential as a drug target in HIV treatment.
At least five isoforms of VPS53 are known to exist.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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