Gibco™

Advanced DMEM/F-12

Advanced DMEM/F-12 (Dulbeccos Modified Eagle Medium/Hams F-12) is a widely used basal medium that allows the culture of mammalian cells자세히 알아보기

Advanced DMEM/F-12 (Dulbeccos Modified Eagle Medium/Hams F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM/F-12, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM/F-12, with no adaptation, include MRC-5, SP2, Vero, WI-38 and Jurkat.

This Advanced DMEM/F-12 is manufactured as follows:

With	Without
• Glucose	• L-glutamine
• Non Essential Amino Acids	• HEPES
• Sodium pyruvate
• Phenol Red

The complete formulation is available.

Using Advanced DMEM/F-12
Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ II lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM/F-12 requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

사양

세포주MRC-5, SP2, Vero, WI-38 and Jurkat

분류Animal Origin

농도1 X

형태Liquid

제품 유형DMEM (Dulbecco`s Modified Eagle Medium)/F-12

Serum LevelReduced Serum

유통 기한12 Months From Date of Manufacture

멸균Sterile-filtered

Sterilization MethodSterile-filtered

첨가제 포함High Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)

첨가제 없음No Glutamine, No HEPES

제조 품질cGMP-compliant under the ISO 13485 standard

제품라인Gibco™

수량500 mL

배송 조건Room Temperature

Unit SizeEach