Thermo Fisher Scientific c-Kit Monoclonal Antibody (1C5)

Applications and Tested Dilution

Product Specifications

Product Specific Information

MA5-15894 targets KIT in indirect ELISA, IHC, and WB applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human KIT expressed in E. coli.
MA5-15894 detects KIT, which has a predicted molecular weight of approximately 145 kDa.

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor).
KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit.
Together with its ligand, KIT regulates growth and activation of various hematopoietic and non-hematopoietic cells.
Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism.

Deregulation of the KIT receptor tyrosine kinase by the prevalent activation loop mutation D816V has become a focal point in therapeutic strategies targeting neoplastic mast cell growth.
c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells, and gastrointestinal tract Cajal cells.

Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of tyrosine residues, including tyrosine 703 on the cytoplasmic domain.
This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway.
SCF/c-kit can activate multiple downstream signaling pathways, including PI3K, PLC-γ, and JAK/STAT.
c-kit receptor activation is essential for hematopoiesis, stem cell maintenance, and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.