Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Alexa Fluor 488, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

Unconjugated, Alexa Fluor 532, Alexa Fluor 561, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV series, Brilliant Violet series, eFluor series, FITC, Functional Grade, NovaFluor series, PE series, PerCP series, Super Bright series, and custom conjugation available.

Product Specific Information

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule (approx. 32–34 kDa). CD8a is expressed as a heterodimer with CD8β (CD8αβ) or as a homodimer (CD8αα). Thymocytes and αβ TCR T cells express CD8αβ, while γδ TCR T cells, intestinal intraepithelial lymphocytes, and dendritic cells express CD8αα. CD8 binds MHC class I and associates with p56lck, playing a role in T cell development and activation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse thymocyte or splenocyte suspensions. Recommended ≤0.5 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Titrate for optimal performance.

Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin family. It plays a key role in signal transduction, T cell differentiation, and activation. CD8 is expressed on T cells as a CD8αβ heterodimer (co-receptor for MHC-I) and as CD8αα homodimers on subsets of T cells, NK cells, monocytes, and dendritic cells. Engagement of MHC-I/peptide complexes triggers Lck recruitment, leading to cytokine production and cytotoxic T lymphocyte (CTL) activation. CTLs are essential for pathogen and tumor cell clearance. Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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