Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Alexa Fluor 488, eBioscience
마우스 CD11c 단백질을 인식하는 N418 단클론 항체로, Alexa Fluor 488 형광 표지. 유세포분석(Flow Cytometry) 및 동결조직 면역염색에 최적화. 고순도 친화 크로마토그래피 정제 및 0.2 µm 여과. 4°C 암소 보관, 동결 금지.
- 카탈로그번호
- 53-0114-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Alexa Fluor 488, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 2 |
| Immunohistochemistry (IHC) | - | 22 |
| Immunohistochemistry (Frozen) (IHC (F)) | 10 µg/mL | 5 |
| Immunocytochemistry (ICC/IF) | - | 8 |
| Flow Cytometry (Flow) | 0.25 µg/test | 126 |
| Miscellaneous PubMed (Misc) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), Alexa Fluor™ 488 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N418 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469902 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 605 / 650 / 711
- eFluor 450 / 506 / 615 / 660
- FITC
- Functional Grade
- NovaFluor Blue 510 / 610-30S / 660-40S / 690
- NovaFluor Yellow 570
- PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 645 / 780
- Request custom conjugation available
Product Specific Information
Description:
The N418 monoclonal antibody reacts with mouse CD11c (integrin alphaX), which associates with beta2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, subsets of intestinal intraepithelial lymphocytes, and some activated T cells. It binds to CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion. N418 recognizes CD11c on splenic dendritic cells and precipitates a 150/90 kDa heterodimer.
Applications Reported:
- Flow cytometric analysis
- Immunohistochemical staining of frozen tissue sections
Applications Tested:
- Flow cytometry: ≤ 0.25 µg/test (100 µL final volume; 10⁵–10⁸ cells/test)
- Immunohistochemistry (frozen tissue): ≤ 10 µg/mL
Careful titration recommended for optimal assay performance.
Fluorescence Characteristics:
- Excitation: 488 nm
- Emission: 519 nm
- Laser: Blue laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD11, together with CD18, forms a heterodimer adhesion molecule.
- CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
- CD11b: Found in monocytes, macrophages, NK cells, some B/T cells, granulocytes
- CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and subsets of lymphocytes
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.