Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Alexa Fluor 488, eBioscience
마우스 CD11c 단백질을 인식하는 N418 단클론 항체로, Alexa Fluor 488 형광 표지. 유세포분석(Flow Cytometry) 및 동결조직 면역염색에 최적화. 고순도 친화 크로마토그래피 정제 및 0.2 µm 여과. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 2 |
| Immunohistochemistry (IHC) | - | 22 |
| Immunohistochemistry (Frozen) (IHC (F)) | 10 µg/mL | 5 |
| Immunocytochemistry (ICC/IF) | - | 8 |
| Flow Cytometry (Flow) | 0.25 µg/test | 126 |
| Miscellaneous PubMed (Misc) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), Alexa Fluor™ 488 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N418 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469902 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 605 / 650 / 711
- eFluor 450 / 506 / 615 / 660
- FITC
- Functional Grade
- NovaFluor Blue 510 / 610-30S / 660-40S / 690
- NovaFluor Yellow 570
- PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 645 / 780
- Request custom conjugation available
Product Specific Information
Description:
The N418 monoclonal antibody reacts with mouse CD11c (integrin alphaX), which associates with beta2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, subsets of intestinal intraepithelial lymphocytes, and some activated T cells. It binds to CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion. N418 recognizes CD11c on splenic dendritic cells and precipitates a 150/90 kDa heterodimer.
Applications Reported:
- Flow cytometric analysis
- Immunohistochemical staining of frozen tissue sections
Applications Tested:
- Flow cytometry: ≤ 0.25 µg/test (100 µL final volume; 10⁵–10⁸ cells/test)
- Immunohistochemistry (frozen tissue): ≤ 10 µg/mL
Careful titration recommended for optimal assay performance.
Fluorescence Characteristics:
- Excitation: 488 nm
- Emission: 519 nm
- Laser: Blue laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD11, together with CD18, forms a heterodimer adhesion molecule.
- CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
- CD11b: Found in monocytes, macrophages, NK cells, some B/T cells, granulocytes
- CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and subsets of lymphocytes
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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