Thermo Fisher Scientific Nestin Monoclonal Antibody (3k1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,500

-

Immunocytochemistry (ICC/IF)

5-10 µg/mL

-

Flow Cytometry (Flow)

2.5 µg per 10^6 cells

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3k1

Immunogen

NS0 cells transfected with a fragment corresponding to residues 618-1618 of human Nestin. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Nestin,uniProtId:P48681-1,ncbiNodeId:9606,antigenRange:618-1618,antigenLength:1621,antigenImageFileName:MA1-26777_Nestin_P48681-1_House_mouse.svg,antigenImageFileNamePDP:MA1-26777_Nestin_P48681-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 5% trehalose

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_795025

Product Specific Information

Recommended positive controls: Human neural progenitors and A172 cells.

MA1-26777 will not react with rodent.

Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot- add 50% glycerol and store at -20ºC or below. Avoid freeze-thaw cycles.

Target Information

Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.