Thermo Fisher Scientific Nestin Monoclonal Antibody (3k1)
인간 Nestin 단백질을 인식하는 Mouse IgG1 단클론 항체로, 신경줄기세포 및 종양 연구에 적합. Western blot, ICC/IF, Flow cytometry에 사용 가능. Rodent 비반응성, 고순도 Protein G 정제, 장기보관 시 -20℃ 권장.
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Thermo Fisher Scientific Nestin Monoclonal Antibody (3k1)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,500 |
| Immunocytochemistry (ICC/IF) | 5–10 µg/mL |
| Flow Cytometry (Flow) | 2.5 µg per 10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3k1 |
| Immunogen | NS0 cells transfected with a fragment corresponding to residues 618–1618 of human Nestin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_795025 |
Product Specific Information
- Recommended positive controls: Human neural progenitors and A172 cells
- MA1-26777 will not react with rodent
- Keep as concentrated solution
- Store at 4ºC short term. For extended storage, aliquot and add 50% glycerol, then store at -20ºC or below
- Avoid freeze-thaw cycles
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells.
It serves as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells.
Nestin is also a superior angiogenic marker for evaluating neovascularity of endothelial cells in tumors.
Highly specific antibodies to human Nestin can aid in:
- Characterizing progenitor cells differentiating into distinct lineages
- Enhancing therapeutic potential of human neural stem/progenitor cells in CNS disease or injury treatment
- Identifying neuroepithelial tumor cells
Nestin expression has been observed in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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