Thermo Fisher Scientific Collagen I Monoclonal Antibody (COL-1)

Thermo Fisher Scientific Collagen I Monoclonal Antibody (COL-1)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Reactive with native (helical) form of collagen type I in ELISA and dot blot assays.
• Not reactive with thermally denatured molecules.
• In immunohistochemical staining of acetone-fixed and unfixed frozen sections, shows strong staining of connective tissue fibers.
• No cross-reactivity with collagen types II, III, IV, V, VI, VII, IX, X, and XI.
• Epitope is sensitive to routine formalin fixation and paraffin embedding.
• Store product as a concentrated solution; centrifuge briefly before opening the vial.

Target Information

Collagen I is a Type I collagen with a triple helix structure composed of two alpha-1 chains and one alpha-2 chain. It is a fibril-forming collagen found in most connective tissues, abundant in bone, cornea, dermis, and tendon.
Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I–IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome.
Symptoms linked to COL1A2 mutations are generally less severe than those in COL1A1, reflecting differing roles in matrix integrity.
Reciprocal translocations between chromosomes 17 and 22, involving Collagen Type I genes and PDGFB, are associated with dermatofibrosarcoma protuberans due to unregulated growth factor expression.
Two transcripts have been identified from alternate polyadenylation signals.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.